478 MODULATION OF HYALURONIC ACID SYNTHESIS BY COLLAGEN HYDROLYSATES IN RABBIT SYNOVIOCYTES (HIG-82)

Electrospinning collagen and hyaluronic acid nanofiber meshes.

Collagen and hyaluronic acid (HA) are main components of the extracellular matrix and have been utilized in electrospinning; a technique that creates nanosized fibers for tissue scaffolds. A collagen/HA polymer solution was electrospun into a scaffold material for osteoporosis patients who have reduced bone strength. To synthesize nanofibers, a high voltage was applied to the polymer solution t...

Hyaluronic Acid Suppresses the Reduction of α2(VI) Collagen Gene Expression Caused by Interleukin-1β in Cultured Rabbit Articular Chondrocytes

Regulation of collagen synthesis by ascorbic acid.

After prolonged exposure to ascorbate, collagen synthesis in cultured human skin fibroblasts increased approximately 8-fold with no significant change in synthesis of noncollagen protein. This effect of ascorbate appears to be unrelated to its cofactor function in collagen hydroxylation. The collagenous protein secreted in the absence of added ascorbate was normal in hydroxylysine but was mildl...

Synthesis and release of hyaluronic acid by Swiss 3T3 fibroblasts.

Hyaluronic acid (HA) and its synthesis were studied in intact Swiss 3T3 mouse fibroblasts and isolated membranes. HA chains in culture medium, attached to cells and in isolated membranes, were determined to possess average M(r) values of 5.2 x 10(6), 1.8 x 10(6) and 0.14 x 10(6) respectively. Log cells were determined to possess 680,000 HA molecules/cell, and to release 120,000 HA chains/h. The...

Interaction between hyaluronic acid and serum dispersed in collagen gels.

A high degree of viscosity is the most striking physical attribute of normal synovial fluid. Although its direct relationship to the lubrication of joints remains uncertain, this viscosity still reflects physiologically important properties of hyaluronic acid. The anomalous viscosity which is a particular feature of hyaluronic acid in synovial fluid depends not only on the concentration and pol...